Cytokeratin 20 Antibody (KRT20/1991)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07285

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KRT20/1991

Concentration

0.2 mg/ml

View all Cytokeratin 20 Antibodies »

Product Summary for Cytokeratin 20 Antibody (KRT20/1991)

Immunogen

Recombinant fragment of human Cytokeratin 20 protein (around aa 196-323) (exact sequence is proprietary) (Uniprot: P35900)

Localization

Cytoplasmic

Specificity

This monoclonal antibody recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions. It has been detected in adenocarcinomas of the colon, stomach and biliary tract. Breast carcinomas are generally non-reactive.

Marker

Colorectal Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08406)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 20 Antibody (KRT20/1991)

Western Blot: Cytokeratin 20 Antibody (KRT20/1991) [NBP3-07285]

Western Blot: Cytokeratin 20 Antibody (KRT20/1991) [NBP3-07285]

Western Blot: Cytokeratin 20 Antibody (KRT20/1991) [NBP3-07285] - Western Blot Analysis of human HT29 cell lysate using Cytokeratin 20 Mouse Monoclonal Antibody (KRT20/1991).
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (KRT20/1991) [NBP3-07285]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (KRT20/1991) [NBP3-07285]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (KRT20/1991) [NBP3-07285] - Formalin-fixed, paraffin-embedded human Normal Colon stained with Cytokeratin 20 Mouse Monoclonal Antibody (KRT20/1991).
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (KRT20/1991) [NBP3-07285]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (KRT20/1991) [NBP3-07285]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (KRT20/1991) [NBP3-07285] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 20 Mouse Monoclonal Antibody (KRT20/1991).
View 1 more images

Applications for Cytokeratin 20 Antibody (KRT20/1991)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 20

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Alternate Names

CD20, CK20, CK-20, Cytokeratin-20, K20cytokeratin 20, keratin 20, keratin, type I cytoskeletal 20, keratin-20, KRT21, MGC35423, Protein IT

Gene Symbol

KRT20

UniProt

P35900 (Human)

Additional Cytokeratin 20 Products

Product Documents for Cytokeratin 20 Antibody (KRT20/1991)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 20 Antibody (KRT20/1991)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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