Cytokeratin 20 Antibody (SA35-03)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67309

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67309
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SA35-03

Concentration

1 mg/ml

View all Cytokeratin 20 Antibodies »

Product Summary for Cytokeratin 20 Antibody (SA35-03)

Immunogen

Synthetic peptide within Human Cytokeratin 20 aa 375-424 / 424. (SwissProt: P35900 Human; SwissProt: P25030 Rat)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 20 Antibody (SA35-03)

Western Blot: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309]

Western Blot: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309]

Western Blot: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of Cytokeratin 20 on CRC cell lysates with Rabbit anti-Cytokeratin 20 antibody at 1/5,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309]

Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Staining Cytokeratin 20 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309]

Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Cytokeratin 20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocke
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Applications for Cytokeratin 20 Antibody (SA35-03)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 20

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Alternate Names

CD20, CK20, CK-20, Cytokeratin-20, K20cytokeratin 20, keratin 20, keratin, type I cytoskeletal 20, keratin-20, KRT21, MGC35423, Protein IT

Gene Symbol

KRT20

Additional Cytokeratin 20 Products

Product Documents for Cytokeratin 20 Antibody (SA35-03)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytokeratin 20 Antibody (SA35-03)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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