Cytokeratin 3/2p Antibody (2Q1040(AE5))
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34844
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Bovine, Rabbit
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2Q1040(AE5)
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Rabbit corneal epithelial keratin
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
Recognizes the 64kD polypeptide/keratin K3 (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Applications for Cytokeratin 3/2p Antibody (2Q1040(AE5))
Application
Recommended Usage
Immunohistochemistry-Frozen
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:500
Application Notes
Immunohistochemistry (Paraffin): 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete's acid-alcohol-formalin following trypsin digestion antigen retrieval.
Formulation, Preparation, and Storage
Purification
Ion exchange chromatography
Formulation
PBS, pH 7.2
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytokeratin 3/2p
Alternate Names
65 KDa Cytokeratin, CK3, CK-3, cytokeratin-3, Keratin, keratin 3, Keratin, Type II Cytoskeletal 3, Keratin-3, Type II Cytoskeletal 3, Type-II keratin Kb3
Gene Symbol
KRT3
UniProt
Additional Cytokeratin 3/2p Products
Product Documents for Cytokeratin 3/2p Antibody (2Q1040(AE5))
Product Specific Notices for Cytokeratin 3/2p Antibody (2Q1040(AE5))
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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