Cytokeratin 5/6 Antibody (KRT5.6/2438)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07628
![Protein Array: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628]](https://resources.bio-techne.com/images/products/Cytokeratin-5-6-Antibody-KRT5-6-2438-Protein-Array-NBP3-07628-img0001.jpg "Protein Array: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628]")
![Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628]](https://resources.bio-techne.com/images/products/Cytokeratin-5-6-Antibody-KRT5-6-2438-Immunohistochemistry-Paraffin-NBP3-07628-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # KRT5.6/2438
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human Cytokeratin 5/6 protein
Localization
Cytoplasmic
Specificity
It recognizes polypeptides of 58kDa and 56kDa, identified as cytokeratin 5 and 6 respectively. It shows no reaction with keratin 1, 8, or 19. It stains the basal cells of squamous and glandular epithelia, part of stratum spinosum of squamous epithelium, myoepithelium, and mesothelium. It does not stain adenous epithelium. Therefore, this antibody can be used to distinguish squamous carcinoma from adenocarcinoma, and to differentiate epitheliod mesothelioma from non-epitheliod mesothelioma and adenocarcinoma. It is also a useful antibody in the identification of mesothelioma and lung squamous cell carcinoma.
Marker
Basal, Myoepithelial & Mesothelial Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08622)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 5/6 Antibody (KRT5.6/2438)
Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628]
Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/2438).![Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628]](https://resources.bio-techne.com/images/products/Cytokeratin-5-6-Antibody-KRT5-6-2438-Immunohistochemistry-Paraffin-NBP3-07628-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628]")
Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628]
Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/2438).![Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628]](https://resources.bio-techne.com/images/products/Cytokeratin-5-6-Antibody-KRT5-6-2438-Immunohistochemistry-Paraffin-NBP3-07628-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628]")
Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628]
Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/2438) [NBP3-07628] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/2438).Applications for Cytokeratin 5/6 Antibody (KRT5.6/2438)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 5/6
Alternate Names
58 kDa cytokeratin, CK5, CK-5, cytokeratin-5, DDD, EBS2, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K5, keratin 5, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes), keratin, type II cytoskeletal 5, Keratin-5, KRT5A, Type-II keratin Kb5
Gene Symbol
KRT5
UniProt
Additional Cytokeratin 5/6 Products
Product Documents for Cytokeratin 5/6 Antibody (KRT5.6/2438)
Product Specific Notices for Cytokeratin 5/6 Antibody (KRT5.6/2438)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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