Cytokeratin 5 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61931

Novus Biologicals, part of Bio-Techne
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NBP2-61931
NBP2-61931SS
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Cytokeratin 5 Antibodies »

Product Specifications

Immunogen

Partial recombinant human Cytokeratin 5 protein (amino acids 19-78)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 5 Antibody - BSA Free

Western Blot: Cytokeratin 5 AntibodyBSA Free [NBP2-61931]

Western Blot: Cytokeratin 5 AntibodyBSA Free [NBP2-61931]

Western Blot: Cytokeratin 5 Antibody [NBP2-61931] - Total protein from human A431 cells and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cytokeratin 5 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody - BSA Free [NBP2-61931]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody - BSA Free [NBP2-61931]

Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody [NBP2-61931] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Triton X-100. The cells were incubated with anti-Cytokeratin 5 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody - BSA Free [NBP2-61931]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody - BSA Free [NBP2-61931]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-61931] - Analysis of a FFPE tissue section of human skin using 1:1000 dilution of rabbit anti-Cytokeratin 5 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Cytokeratin 5 antibody generated a specific membrane cytoplasmic staining in the epithelial cells of the skin tissue. The signal was highest in the keratinocytes while dermis was largely negative for this Cytokeratin 5 (KRT5).
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Applications for Cytokeratin 5 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

2-10 ug/million cells

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 5

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Alternate Names

CK-5, DDD, EBS2, Keratin 5, KRT5

Gene Symbol

KRT5

Additional Cytokeratin 5 Products

Product Documents for Cytokeratin 5 Antibody - BSA Free

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Product Specific Notices for Cytokeratin 5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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