Cytokeratin 6a Antibody (KRT6/3997R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14107
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # KRT6/3997R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Cytokeratin 6a Antibody (KRT6/3997R) - Azide and BSA Free
Immunogen
Recombinant fragment of human Cytokeratin 6a (exact sequence is proprietary) (Uniprot: P02538)
Localization
Cytoplasm
Marker
Basal Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13955).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Cytokeratin 6a Antibody (KRT6/3997R) - Azide and BSA Free
Cytokeratin 6a Antibody (KRT6/3997R)
Formalin-fixed, paraffin-embedded human bladder carcinoma pT24 stained with Cytokeratin 6a antibody (KRT6/3997R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Cytokeratin 6a Antibody (KRT6/3997R)
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using Cytokeratin 6a antibody (KRT6/3997R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Cytokeratin 6a Antibody (KRT6/3997R)
Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with Cytokeratin 6a antibody (KRT6/3997R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Cytokeratin 6a Antibody (KRT6/3997R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Cytokeratin 6a
Alternate Names
Allergen Hom s 5, CK6A, CK-6A, CK6C, CK6D, CK-6D, cytokeratin 6A, cytokeratin 6C, cytokeratin 6D, Cytokeratin-6A, Cytokeratin-6D, K6A, K6C, K6D, keratin 6A, keratin 6C, keratin 6D, keratin, epidermal type II, K6A, keratin, type II cytoskeletal 6A, Keratin-6A, KRT6C, KRT6D, Type-II keratin Kb6
Gene Symbol
KRT6A
Additional Cytokeratin 6a Products
Product Documents for Cytokeratin 6a Antibody (KRT6/3997R) - Azide and BSA Free
Product Specific Notices for Cytokeratin 6a Antibody (KRT6/3997R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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