Cytokeratin 8/18 Antibody (5D3)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-17139
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
IF/IHC, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5D3
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Cytokeratins from the human breast carcinoma cell line MCF-7.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 11277418). Not yet tested in other species.
Localization
Cytoplasmic
Specificity
This antibody is specific to human cytokeratins 8 (52.5 kDa) and 18 (45 kDa). It stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Cytokeratin 8/18 Antibody (5D3)
![Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (5D3) [NB120-17139]](https://resources.bio-techne.com/images/products/Cytokeratin-8-18-Antibody-5D3-Immunohistochemistry-Paraffin-NB120-17139-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (5D3) [NB120-17139]")
Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (5D3) [NB120-17139]
Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (5D3) [NB120-17139] - Formalin fixed paraffin embedded human skin stained with Cytokeratin 8/18 antibody (NB120-17139).Applications for Cytokeratin 8/18 Antibody (5D3)
Application
Recommended Usage
Immunohistochemistry
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:100
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Tissue culture supernatant
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 8/18
Alternate Names
CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
Entrez Gene IDs
3856 (Human)
Gene Symbol
KRT8
Additional Cytokeratin 8/18 Products
Product Documents for Cytokeratin 8/18 Antibody (5D3)
Product Specific Notices for Cytokeratin 8/18 Antibody (5D3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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