Cytokeratin 8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14177

Novus Biologicals, part of Bio-Techne
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NBP2-14177
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 8 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK

Marker

Epithelial Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 8 Antibody

Western Blot: Cytokeratin 8 Antibody [NBP2-14177]

Western Blot: Cytokeratin 8 Antibody [NBP2-14177]

Western Blot: Cytokeratin 8 Antibody [NBP2-14177] - Analysis in human cell lines MCF-7 and SK-MEL-30 using anti-KRT8 antibody. Corresponding KRT8 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody [NBP2-14177]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody [NBP2-14177]

Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody [NBP2-14177] - Staining of human cell line MCF7 shows positivity in intermediate filaments.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-14177]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-14177]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-14177] - Staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.
View 1 more images

Applications for Cytokeratin 8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 8

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Alternate Names

CARD2, CK8, EndoA, KRT8

Gene Symbol

KRT8

Additional Cytokeratin 8 Products

Product Documents for Cytokeratin 8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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