Key Product Details

Species Reactivity

Human, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # KRTH/1576R

Concentration

0.2 mg/ml

View all Cytokeratin, HMW Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Cytokeratin, HMW protein (Uniprot: Q01546)

Reactivity Notes

Expected to show broad species reactivity.

Localization

Cytoplasmic

Specificity

This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

200ug/ml of recombinant monoclonal antibody purified by Protein A or G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. (NBP2-54589)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin, HMW Antibody (KRTH/1576R)

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/1576R) [NBP2-53164]

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/1576R) [NBP2-53164]

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/1576R) [NBP2-53164] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Antibody (KRTH/1576R).
Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/1576R) [NBP2-53164]

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/1576R) [NBP2-53164]

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/1576R) [NBP2-53164] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Recombinant Rabbit Monoclonal Antibody (KRTH/1576R).

Applications for Cytokeratin, HMW Antibody (KRTH/1576R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin, HMW

KRT76, also known as Keratin, type II cytoskeletal 2 oral, is a 638 amino acid that is approx. 66 kDa, belongs to the intermediate filament family responsible for the structural integrity of epithelial cells, subdivided into epithelial keratins and hair keratins, and probably contributes to terminal cornification. Studies are being performed in relation to this protein and obstructive jaundice, pharyngitis, jaundice, cervical cancer, neuronitis, colorectal cancer, cervicitis, interferon and hepatitis. This protein has been shown to have interactions with ATG13, PDPK1, ULK2, GABARAP, and PRKAA1 in cytoskeleton organization pathways.

Alternate Names

CK-2P, cytokeratin 2, Cytokeratin-2P, HUMCYT2A, K2P, K76, keratin 76, keratin, type II cytoskeletal 2 oral, keratin-76, KRT2Bcytokeratin-2P, KRT2Pkeratin 2p, KRT76, Type-II keratin Kb9

Gene Symbol

KRT76

UniProt

Q01546 (Human)

Additional Cytokeratin, HMW Products

Product Documents for Cytokeratin, HMW Antibody (KRTH/1576R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin, HMW Antibody (KRTH/1576R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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