Cytokeratin, HMW Antibody (KRTH/2147R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07272

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-07272-100ug
NBP3-07272-20ug
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # KRTH/2147R

Concentration

0.2 mg/ml

View all Cytokeratin, HMW Antibodies »

Product Specifications

Immunogen

Solubilized keratin extract from human stratum corneum

Localization

Cytoplasmic

Specificity

This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This monoclonal antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08361)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin, HMW Antibody (KRTH/2147R)

Immunocytochemistry/ Immunofluorescence: Cytokeratin, HMW Antibody (KRTH/2147R) [NBP3-07272]

Immunocytochemistry/ Immunofluorescence: Cytokeratin, HMW Antibody (KRTH/2147R) [NBP3-07272]

Immunocytochemistry/Immunofluorescence: Cytokeratin, HMW Antibody (KRTH/2147R) [NBP3-07272] - Immunofluorescence Analysis of MeOH-fixed Human MCF-7 cells labeling CK with Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/2147R) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).
Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/2147R) [NBP3-07272]

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/2147R) [NBP3-07272]

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (KRTH/2147R) [NBP3-07272] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin, HMW Rabbit Recombinant Monoclonal Antibody (KRTH/2147R).

Applications for Cytokeratin, HMW Antibody (KRTH/2147R)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin, HMW

KRT76, also known as Keratin, type II cytoskeletal 2 oral, is a 638 amino acid that is approx. 66 kDa, belongs to the intermediate filament family responsible for the structural integrity of epithelial cells, subdivided into epithelial keratins and hair keratins, and probably contributes to terminal cornification. Studies are being performed in relation to this protein and obstructive jaundice, pharyngitis, jaundice, cervical cancer, neuronitis, colorectal cancer, cervicitis, interferon and hepatitis. This protein has been shown to have interactions with ATG13, PDPK1, ULK2, GABARAP, and PRKAA1 in cytoskeleton organization pathways.

Alternate Names

CK-2P, cytokeratin 2, Cytokeratin-2P, HUMCYT2A, K2P, K76, keratin 76, keratin, type II cytoskeletal 2 oral, keratin-76, KRT2Bcytokeratin-2P, KRT2Pkeratin 2p, KRT76, Type-II keratin Kb9

Gene Symbol

KRT76

UniProt

Q01546 (Human)

Additional Cytokeratin, HMW Products

Product Documents for Cytokeratin, HMW Antibody (KRTH/2147R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin, HMW Antibody (KRTH/2147R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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