Cytokeratin, LMW Antibody (rKRTL/8751)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23655
Recombinant Monoclonal Antibody
![Cytokeratin, LMW Antibody (rKRTL/8751) Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/8751) [NBP3-23655] -](https://resources.bio-techne.com/images/products/nbp3-23655_mouse-cytokeratin-lmw-mab-rkrtl-8751-2412025863676.jpg "Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/8751) [NBP3-23655] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit, Reptile
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rKRTL/8751
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human epidermal keratin
Localization
Cytoplasm.
Specificity
This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Store at 2 to 8C. Non-hazardous. No MSDS required.
Scientific Data Images for Cytokeratin, LMW Antibody (rKRTL/8751)
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/8751) [NBP3-23655] -
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (rKRTL/8751) [NBP3-23655] - Formalin-fixed, paraffin-embedded human placenta stained with Cytokeratin, LMW Antibody (rKRTL/8751). Inset: PBS instead of primary antibody; secondary only negative control.Applications for Cytokeratin, LMW Antibody (rKRTL/8751)
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry-Paraffin: 0.5-1ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin, LMW
Alternate Names
Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39
Gene Symbol
KRT77
Additional Cytokeratin, LMW Products
Product Documents for Cytokeratin, LMW Antibody (rKRTL/8751)
Product Specific Notices for Cytokeratin, LMW Antibody (rKRTL/8751)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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