DAP Kinase 1 Antibody (3E10G3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15382

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15382-100ul
NBP3-15382-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3E10G3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DAP Kinase 1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human DAP Kinase 1 (P53355). DFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DAP Kinase 1 Antibody (3E10G3)

Western Blot: DAP Kinase 1 Antibody (3E10G3) [NBP3-15382]

Western Blot: DAP Kinase 1 Antibody (3E10G3) [NBP3-15382]

Western Blot: DAP Kinase 1 Antibody (3E10G3) [NBP3-15382] - Western blot analysis of extracts of various cell lines, using DAP Kinase 1 Rabbit mAb (NBP3-15382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for DAP Kinase 1 Antibody (3E10G3)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DAP Kinase 1

DAPK1 (Death-associated protein kinase 1) is a calmodulin dependent serine/threonine serine kinase which functions as a positive mediator of gamma-interferon induced programmed cell death. DAPK1 expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlates with high rates of metastasis. The loss of DAPK expression provides a link between suppression of apoptosis and metastasis. DAPK1 is thought be involved in an early apoptotic checkpoint which eliminates premalignant cells from cancer formation. Studies in bladder cancer patients have also shown that hypermethylation of DAPK1 correlates to high recurrence rates and thus DAPK1 may be used as a prognostic marker. DAPK1 is also reportedly a molecular regulator of neuronal death in epilepsy.

Long Name

Death-associated protein kinase 1

Alternate Names

DAPK, DAPK1

Gene Symbol

DAPK1

Additional DAP Kinase 1 Products

Product Documents for DAP Kinase 1 Antibody (3E10G3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DAP Kinase 1 Antibody (3E10G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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