DAP Kinase 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57307

Novus Biologicals, part of Bio-Techne
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NBP2-57307
NBP2-57307-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DAPK2 Antibodies »

Product Summary for DAP Kinase 2 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: MFQASMRSPNMEPFKQQKVEDFYDIGEEL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DAP Kinase 2 Antibody

Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody [NBP2-57307]

Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody [NBP2-57307]

Immunocytochemistry/Immunofluorescence: DAP Kinase 2 Antibody [NBP2-57307] - Staining of human cell line HaCaT shows localization to the Golgi apparatus & vesicles.

Applications for DAP Kinase 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAPK2

Protein kinase termed death-associated protein kinase 2 (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling (1). The region of homology spans the catalytic domain and the CaM-regulatory region, whereas the remaining C-terminal part of the protein differs completely from DAP kinase and displays no homology to any known protein. The catalytic domain is also homologous to the recently identified ZIP kinase and to a lesser extent to the catalytic domains of DRAK1 and -2 (2). DAPK2 has a novel regulatory mechanism that controls its pro-apoptotic functions. It comprises a single autophosphorylation event mapped to Ser308 within the CaM regulatory domain. A negative charge at this site reduces both the binding to CaM and the formation of DRP-1 homodimers (3).

Long Name

Death-associated Protein Kinase 2

Alternate Names

DAP Kinase 2, DRP-1

Gene Symbol

DAPK2

Additional DAPK2 Products

Product Documents for DAP Kinase 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DAP Kinase 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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