Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DAP1 Antibodies »

Product Summary for DAP1 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 52 and 102 of human Death-Associated Protein using the numbering given in entry NP_004385.1 (GeneID 1611).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DAP1 Antibody

Western Blot: DAP1 Antibody [NBP2-22259]

Western Blot: DAP1 Antibody [NBP2-22259]

Western Blot: DAP1 Antibody [NBP2-22259] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. NBP2-22259 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: DAP1 Antibody [NBP2-22259]

Immunoprecipitation: DAP1 Antibody [NBP2-22259]

Immunoprecipitation: DAP1 Antibody [NBP2-22259] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: NBP2-22259 used for IP at 6 ug/mg lysate. DAP1 was also immunoprecipitated by another rabbit anti-DAP1 antibody. For blotting immunoprecipitated DAP1, NBP2-22259 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for DAP1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:500 - 1:2500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DAP1

Death associated protein 1 (DAP-1) is a 15-kDa protein that functions as a positive mediator of cell death initiated by interferon (IFN-) gamma (1,2). DAP-1 is proline rich and possesses one SH3 binding motif, as well as several consensus protein kinase phosphorylation sites (1). It is localized to the cytoplasm, but the detailed mechanism of its pro-apoptotic function is unclear. DAP-1 and DAP kinase, a unique, 160-kDa calmodulin-dependent serine/threonine kinase, are both unique candidates for positive mediators of programmed cell death induced by IFN-gamma (3).

Alternate Names

DAP1, DAP-1, death-associated protein, death-associated protein 1, MGC99796

Gene Symbol

DAP

UniProt

NP_004385.1 (Human)

Additional DAP1 Products

Product Documents for DAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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