DAP1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92897

Novus Biologicals, part of Bio-Techne
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NBP2-92897-0.02ml
NBP2-92897-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DAP1 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human DAP (NP_004385.1). MSSPPEGKLETKAGHPPAVKAGGMRIVQKHPHTGDTKEEKDKDDQEWESPSPPKPTVFISGVIARGDKDFPPAAAQVAHQKPHASMDKHPSPRTQHIQQPRK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAP1 Antibody - BSA Free

Western Blot: DAP1 AntibodyBSA Free [NBP2-92897]

Western Blot: DAP1 AntibodyBSA Free [NBP2-92897]

Western Blot: DAP1 Antibody [NBP2-92897] - Analysis of extracts of various cell lines, using DAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: DAP1 Antibody - BSA Free [NBP2-92897]

Immunocytochemistry/ Immunofluorescence: DAP1 Antibody - BSA Free [NBP2-92897]

Immunocytochemistry/Immunofluorescence: DAP1 Antibody [NBP2-92897] - Analysis of MCF-7 cells using DAP1 . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: DAP1 Antibody - BSA Free [NBP2-92897]

Immunohistochemistry-Paraffin: DAP1 Antibody - BSA Free [NBP2-92897]

Immunohistochemistry-Paraffin: DAP1 Antibody [NBP2-92897] - Rat kidney using DAP antibody at dilution of 1:200 (40x lens).

Applications for DAP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DAP1

Death associated protein 1 (DAP-1) is a 15-kDa protein that functions as a positive mediator of cell death initiated by interferon (IFN-) gamma (1,2). DAP-1 is proline rich and possesses one SH3 binding motif, as well as several consensus protein kinase phosphorylation sites (1). It is localized to the cytoplasm, but the detailed mechanism of its pro-apoptotic function is unclear. DAP-1 and DAP kinase, a unique, 160-kDa calmodulin-dependent serine/threonine kinase, are both unique candidates for positive mediators of programmed cell death induced by IFN-gamma (3).

Alternate Names

DAP1, DAP-1, death-associated protein, death-associated protein 1, MGC99796

Gene Symbol

DAP

Additional DAP1 Products

Product Documents for DAP1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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