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DAP12 Antibody (SR3887)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27816

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-27816-100ul
NBP3-27816-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR3887

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for DAP12 Antibody (SR3887)

Immunogen

A synthesized peptide derived from human DAP12 (Uniprot #: O43914)

Specificity

Detects endogenous levels of total DAP12

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAP12 Antibody (SR3887)

Western Blot: DAP12 Antibody (SR3887) [NBP3-27816] -

Western Blot: DAP12 Antibody (SR3887) [NBP3-27816] - Western blot analysis of DAP12 expression in Human PBMC cell lysate.

Applications for DAP12 Antibody (SR3887)

Application
Recommended Usage

Flow Cytometry

1:80

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAP12

DAP12 is a transmembrane adaptor protein that is non-covalently associated with several cell surface receptors on natural killer (NK), myeloid, and presumably neuronal cells. DAP12 contains an ITAM domain that preferentially recruits the protein tyrosine kinase Syk to initiate signal transduction. Deficiency of DAP12 results in reduced antigen presentation function by myeloid cells and defects in function of certain NK cell receptors in mice, as well as presenile dementia and bone cysts in humans.

Long Name

DNAX-activation Protein 12

Alternate Names

KARAP, TYROBP

Gene Symbol

TYROBP

Additional DAP12 Products

Product Documents for DAP12 Antibody (SR3887)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DAP12 Antibody (SR3887)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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