DAP3 Antibody (OTI1B12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71338

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DAP3 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 161-398 of human DAP3 (NP_387506) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAP3 Antibody (OTI1B12) - Azide and BSA Free

Western Blot: DAP3 Antibody (OTI1B12)Azide and BSA Free [NBP2-71338]

Western Blot: DAP3 Antibody (OTI1B12)Azide and BSA Free [NBP2-71338]

Western Blot: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3.
Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338]

Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338]

Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338]

Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338]

Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for DAP3 Antibody (OTI1B12) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DAP3

Death Associated Protein 3 (DAP3) is a 46 kDa protein carrying a potential nucleotide binding motif (P-loop) at its N-terminal region. The protein is widely expressed, and the expression is upregulated during membrane-receptor mediated apoptosis. In interferon-gamma and Fas induced apoptosis, DAP3 acts as a positive mediator by functioning downstream of the receptor signaling complex and upstream of the effector caspases. However, the mechanism for its function is largely unknown. Interestingly, the N-terminal 230 amino acid fragment of DAP3 alone protects cells from Fas induced cell death. The same region of DAP3 also serves as a binding site for the glucocorticoid receptor and mediates receptor functions.

Alternate Names

bMRP-10, DAP-3Death-associated protein 3, death associated protein 3, DKFZp686G12159,28S ribosomal protein S29, mitochondrial, FLJ12817, MGC126058, MGC126059, mitochondrial 28S ribosomal protein S29, MRP-S29Ionizing radiation resistance conferring protein, MRPS29S29mt

Gene Symbol

DAP3

Additional DAP3 Products

Product Documents for DAP3 Antibody (OTI1B12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DAP3 Antibody (OTI1B12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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