DARPP-32 Antibody (2G6D9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37288

Novus Biologicals, part of Bio-Techne
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NBP2-37288
NBP2-37288-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2G6D9

Format

BSA Free

Concentration

1 mg/ml

View all DARPP-32 Antibodies »

Product Summary for DARPP-32 Antibody (2G6D9) - BSA Free

Immunogen

Purified recombinant fragment of human DARPP-32 (AA: 95-204) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DARPP-32 Antibody (2G6D9) - BSA Free

Western Blot: DARPP-32 Antibody (2G6D9)BSA Free [NBP2-37288]

Western Blot: DARPP-32 Antibody (2G6D9)BSA Free [NBP2-37288]

Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against HEK293 (1) and PPP1R1B (AA: 95-204)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: DARPP-32 Antibody (2G6D9) - BSA Free [NBP2-37288]

Flow Cytometry: DARPP-32 Antibody (2G6D9) - BSA Free [NBP2-37288]

Flow Cytometry: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Flow cytometric analysis of Hela cells using PPP1R1B mouse mAb (green) and negative control (red).
Western Blot: DARPP-32 Antibody (2G6D9)BSA Free [NBP2-37288]

Western Blot: DARPP-32 Antibody (2G6D9)BSA Free [NBP2-37288]

Western Blot: DARPP-32 Antibody (2G6D9) [NBP2-37288] - Western blot analysis using PPP1R1B mAb against human PPP1R1B (AA: 95-204) recombinant protein. (Expected MW is 38.3 kDa)
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Applications for DARPP-32 Antibody (2G6D9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DARPP-32

DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac

Long Name

Dopamine and cAMP-regulated Phosphoprotein

Alternate Names

DARPP32, PPP1R1B

Gene Symbol

PPP1R1B

Additional DARPP-32 Products

Product Documents for DARPP-32 Antibody (2G6D9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DARPP-32 Antibody (2G6D9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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