DARPP-32 Antibody (5T9G4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16316

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16316-100ul
NBP3-16316-20ul
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5T9G4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DARPP-32 Antibodies »

Product Summary for DARPP-32 Antibody (5T9G4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DARPP-32 (Q9UD71). MDPKDRKKIQFSVPAPPSQLDPRQVEMIRRRRPTPAMLFRLSEHSSPEEEASPHQRASGEGHHLKSKRPNPCAYTPPSLKAVQRIAESHLQSISNLNENQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DARPP-32 Antibody (5T9G4)

Western Blot: DARPP-32 Antibody (5T9G4) [NBP3-16316]

Western Blot: DARPP-32 Antibody (5T9G4) [NBP3-16316]

Western Blot: DARPP-32 Antibody (5T9G4) [NBP3-16316] - Western blot analysis of extracts of various cell lines, using DARPP-32 Rabbit mAb (NBP3-16316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for DARPP-32 Antibody (5T9G4)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DARPP-32

DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac

Long Name

Dopamine and cAMP-regulated Phosphoprotein

Alternate Names

DARPP32, PPP1R1B

Gene Symbol

PPP1R1B

Additional DARPP-32 Products

Product Documents for DARPP-32 Antibody (5T9G4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DARPP-32 Antibody (5T9G4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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