DARPP-32 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-304

Novus Biologicals, part of Bio-Techne
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NB300-304
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DARPP-32 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # Q6J4I0

Reactivity Notes

It is anticipated that the antibody will also react with bovine, canine, chicken, mouse, non-human primate and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Specificity

Specific for endogenous levels of the ~32 kDa DARPP-32 protein.

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DARPP-32 Antibody

Western Blot: DARPP-32 Antibody [NB300-304]

Western Blot: DARPP-32 Antibody [NB300-304]

Western Blot: DARPP32 Antibody [NB300-304] - Detection of DARPP protein in rat hippocampal lysate using NB 300-304.
Immunocytochemistry/ Immunofluorescence: DARPP-32 Antibody [NB300-304]

Immunocytochemistry/ Immunofluorescence: DARPP-32 Antibody [NB300-304]

Immunocytochemistry/Immunofluorescence: DARPP-32 Antibody [NB300-304] - Staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32 in green and Tuj1 in red. Cells and photo courtesy of QBMCellScience.

Applications for DARPP-32 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DARPP-32

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP- 2 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac

Long Name

Dopamine and cAMP-regulated Phosphoprotein

Alternate Names

DARPP32, PPP1R1B

Entrez Gene IDs

84152 (Human); 19049 (Mouse); 360616 (Rat)

Gene Symbol

PPP1R1B

UniProt

Q6J4I0 (Rat)

Additional DARPP-32 Products

Product Documents for DARPP-32 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DARPP-32 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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