DAX1/NR0B1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32832
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Chimpanzee (99%), Rhesus Macaque (95%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western
Cited:
Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human DAX1/NR0B1. The exact sequence is proprietary.
Localization
Nucleus, Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DAX1/NR0B1 Antibody
![Immunocytochemistry/ Immunofluorescence: DAX1/NR0B1 Antibody [NBP1-32832]](https://resources.bio-techne.com/images/products/DAX1-NR0B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32832-img0008.jpg "Immunocytochemistry/ Immunofluorescence: DAX1/NR0B1 Antibody [NBP1-32832]")
Immunocytochemistry/ Immunofluorescence: DAX1/NR0B1 Antibody [NBP1-32832]
Immunocytochemistry/Immunofluorescence: DAX1/NR0B1 Antibody [NBP1-32832] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NR0B1 protein stained by NR0B1 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.![Immunohistochemistry-Paraffin: DAX1/NR0B1 Antibody [NBP1-32832]](https://resources.bio-techne.com/images/products/DAX1-NR0B1-Antibody-Immunohistochemistry-Paraffin-NBP1-32832-img0007.jpg "Immunohistochemistry-Paraffin: DAX1/NR0B1 Antibody [NBP1-32832]")
Immunohistochemistry-Paraffin: DAX1/NR0B1 Antibody [NBP1-32832]
Immunohistochemistry-Paraffin: DAX1/NR0B1 Antibody [NBP1-32832] - Cal27 xenograft, using NR0B1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for DAX1/NR0B1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Simple Western
reported by internal validation
Application Notes
In Simple Western internal validation: Human fetal adrenal glands as sample; separated by size; antibody dilution of 1:50; observed molecular weight was ~51 kDa;matrix was 12-230 kDa; detected by Chemiluminescence.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: DAX1/NR0B1
Long Name
Orphan Nuclear Receptor DAX1/Nuclear Receptor Subfamily 0, Group B, Member 1
Alternate Names
AHC, AHCH, NR0B1
Entrez Gene IDs
190 (Human)
Gene Symbol
NR0B1
UniProt
Additional DAX1/NR0B1 Products
Product Documents for DAX1/NR0B1 Antibody
Product Specific Notices for DAX1/NR0B1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.