Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # DAXX-01

Format

BSA Free

Concentration

1.0 mg/ml

View all Daxx Antibodies »

Product Summary for Daxx Antibody (DAXX-01) - BSA Free

Immunogen

Recombinant C-terminal part (aa 558-740) of human Daxx.

Specificity

The antibody DAXX-01 reacts with Daxx, a death domain containing protein mainly expressed in fetal and adult human and mouse tissue.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Daxx Antibody (DAXX-01) - BSA Free

Immunocytochemistry/ Immunofluorescence: Daxx Antibody (DAXX-01) - BSA Free [NB500-491]

Immunocytochemistry/ Immunofluorescence: Daxx Antibody (DAXX-01) - BSA Free [NB500-491]

Immunocytochemistry/Immunofluorescence: Daxx Antibody (DAXX-01) [NB500-491] - 1B - HeLa cells were co-transfected with pCDNA3-MycDaxx and pCDNA3-ASK1HA, which led to translocation of Daxx from nucleus to cytoplasm
Immunocytochemistry/ Immunofluorescence: Daxx Antibody (DAXX-01) - BSA Free [NB500-491]

Immunocytochemistry/ Immunofluorescence: Daxx Antibody (DAXX-01) - BSA Free [NB500-491]

Immunocytochemistry/Immunofluorescence: Daxx Antibody (DAXX-01) [NB500-491] - Fig. 1. Immunofluorescence staining of Daxx in transfected HeLa human cervix carcinoma cell line. Myc Daxx (green) was stained with anti-human Daxx (DAXX-01), nuclei were stained with DAPI (blue). 1A - nuclear localization of Daxx in HeLa cells transfected with pCDNA3-MycDaxx.

Applications for Daxx Antibody (DAXX-01) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Daxx

Daxx is an apoptosis-modulating death domain-associated protein with functions in transcriptional regulation. Daxx functions bot in cytoplasm, where it interacts with Fas, and in nucleus (residing in PML oncogenic domains), where it is involved in SUMO-dependent regulation of transcription and subnuclear compartmentalization. Daxx senzitizes the cells to apoptosis, but on the other hand, this protein may also serve in preventing apoptosis in the early embryo. Even regarding the transcription, Daxx can serve both as a corepressor and a coactivator. During ischemic stress, Daxx translocates from the nucleus to the cytoplasm, where in regulates sodium hydrogen exchanger isoform 1 (NHE1).

Long Name

Fas Death Domain-associated Protein

Alternate Names

BING2, DAP6

Entrez Gene IDs

1616 (Human)

Gene Symbol

DAXX

Additional Daxx Products

Product Documents for Daxx Antibody (DAXX-01) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Daxx Antibody (DAXX-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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