Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08741

Novus Biologicals, part of Bio-Techne
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NBP3-08741
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # PCRP-DAXX-8C2

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Daxx Antibodies »

Product Summary for Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free

Immunogen

Recombinant full-length human Daxx protein (Uniprot: Q9UER7)

Localization

Nucleus. Cytosol.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

81.37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07839).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free [NBP3-08741]

Immunocytochemistry/ Immunofluorescence: Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free [NBP3-08741]

Immunocytochemistry/Immunofluorescence: Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free [NBP3-08741] - Immunofluorescence Analysis of PFA-fixed HeLa cells using Daxx Mouse Monoclonal Antibody (PCRP-DAXX-8C2) followed by goat anti- Mouse IgG-CF488 (green).
Immunohistochemistry-Paraffin: Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free [NBP3-08741]

Immunohistochemistry-Paraffin: Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free [NBP3-08741]

Immunohistochemistry-Paraffin: Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free [NBP3-08741] - Formalin-fixed, paraffin-embedded human kidney stained with Daxx Mouse Monoclonal Antibody (PCRP-Daxx-8C2).
Flow Cytometry: Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free [NBP3-08741]

Flow Cytometry: Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free [NBP3-08741]

Flow Cytometry: Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free [NBP3-08741] - Flow cytometric analysis of PFA-fixed HeLa cells. Daxx Mouse Monoclonal Antibody (PCRP-Daxx-8C2) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Applications for Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Daxx

Daxx (death-domain-associated protein) was originally identified as a cytoplasmic protein that binds to the death domain of the transmembrane death receptor Fas. It is now that known that the a large porportion of Daxx molecules are nuclear and associate with the promyelocytic leukaemia nuclear body (PML-NB) and other subnuclear domains (reviewed in Salomoni and Khelifi). The promyelocytic leukemia protein Pml is an essential component of the PML-NB. Data suggests that certain stimuli including Fas stimulation, causes Daxx to be translocated from the nucleus to the cytoplasm. Daxx is ubiquitously expressed, and particularly high levels of Daxx have been reported in the thymus and testes. At the cellular level Daxx may be found in cytoplasm and within heterochromatic regions of the nucleus. Daxx has been reported to interact and co-localize with Pml in the nucleus of tumor cell lines and primary cells. Pml is necessary for Fas-induced cell death and Daxx pro-apoptotic functions. Daxx is thought to have both pro- and anti-apoptotic functions depending on the stimulus and the cell type. Daxx pro-apoptotic functions appear to occur in both the cytoplasm and nucleus. Although the mechanisms remain to be fully elucidated, research indicates that Daxx plays a role in the pathology of human diseases including cancer and neurodegerative disorders. Human Daxx is a 740 amino acid protein (GenBank no. gi/62898369).

Long Name

Fas Death Domain-associated Protein

Alternate Names

BING2, DAP6

Gene Symbol

DAXX

Additional Daxx Products

Product Documents for Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Daxx Antibody (PCRP-DAXX-8C2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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