Daxx Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56136

Novus Biologicals, part of Bio-Techne
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NB100-56136
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunoblotting, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Daxx Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 272-291 (ERLINKPGPDTFPDYGDVLR, GenBank no. gi/62898369) of human DAXX was used as immuogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Daxx Antibody

Immunohistochemistry-Paraffin: Daxx Antibody [NB100-56136]

Immunohistochemistry-Paraffin: Daxx Antibody [NB100-56136]

Immunohistochemistry-Paraffin: Daxx Antibody - Unpurified [NB100-56136] - Daxx Antibody [NB100-56136] - Tissue sections of human prostate cancer (A and B) stained for Daxx expression using this antibody at 1:2000. Hematoxylin-eosin counterstain.

Applications for Daxx Antibody

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 28501693)

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Daxx

DAXX (death-domain-associated protein) was originally identified as a cytoplasmic protein that binds to the death domain of the transmembrane death receptor Fas. It is now that known that the a large proportion of DAXX molecules are nuclear and associate with the promyelocytic leukemia nuclear body (PML-NB) and other subnuclear domains (reviewed in Salomoni and Khelifi). The promyelocytic leukemia protein PML is an essential component of the PML-NB. Data suggests that certain stimuli including Fas stimulation, causes DAXX to be translocated from the nucleus to the cytoplasm. DAXX is ubiquitously expressed, and particularly high levels of DAXX have been reported in the thymus and testes. At the cellular level DAXX may be found in cytoplasm and within heterochromatic regions of the nucleus. DAXX has been reported to interact and co-localize with PML in the nucleus of tumor cell lines and primary cells. PML is necessary for Fas-induced cell death and DAXX pro-apoptotic functions. DAXX is thought to have both pro- and anti-apoptotic functions depending on the stimulus and the cell type. DAXX pro-apoptotic functions appear to occur in both the cytoplasm and nucleus. Although the mechanisms remain to be fully elucidated, research indicates that DAXX plays a role in the pathology of human diseases including cancer and neurodegenerative disorders. Human DAXX is a 740 amino acid protein (GenBank no. gi/62898369).

Long Name

Fas Death Domain-associated Protein

Alternate Names

BING2, DAP6

Entrez Gene IDs

1616 (Human); 13163 (Mouse); 140926 (Rat)

Gene Symbol

DAXX

Additional Daxx Products

Product Documents for Daxx Antibody

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Certificate of Analysis

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Product Specific Notices for Daxx Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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