DAZL Antibody (DAZL/4253)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07840

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Cynomolgus Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # DAZL/4253

Concentration

0.2 mg/ml

View all DAZL Antibodies »

Product Specifications

Immunogen

Recognizes DAZL protein C terminus sequence CRVHHFRRSRAMLKSV. (Uniprot: Q92904)

Localization

Cytoplasmic

Specificity

Specific for human DAZL, a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localized to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Clone DAZL/4253 recognizes DAZL at its C terminus, and is used in detection of DAZL expression by various methods.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08742)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for DAZL Antibody (DAZL/4253)

Western Blot: DAZL Antibody (DAZL/4253) [NBP3-07840]

Western Blot: DAZL Antibody (DAZL/4253) [NBP3-07840]

Western Blot: DAZL Antibody (DAZL/4253) [NBP3-07840] - Western blot Analysis testis tissue lysate using DAZL Mouse Monoclonal Antibody (DAZL/4253).
Immunohistochemistry-Paraffin: DAZL Antibody (DAZL/4253) [NBP3-07840]

Immunohistochemistry-Paraffin: DAZL Antibody (DAZL/4253) [NBP3-07840]

Immunohistochemistry-Paraffin: DAZL Antibody (DAZL/4253) [NBP3-07840] - Formalin-fixed, paraffin-embedded human testis stained with DAZL Mouse Monoclonal Antibody (DAZL/4253).

Applications for DAZL Antibody (DAZL/4253)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: DAZL

DAZL is a RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts

Alternate Names

DAZHSPGY-like-autosomal, DAZL1DAZ-like autosomal, DAZLA, deleted in azoospermia-like, Deleted in azoospermia-like 1, germline specific RNA binding protein, MGC26406, spermatogenesis gene on the Y-like autosomal, SPGYLADAZ homolog

Gene Symbol

DAZL

UniProt

Q92904 (Human)

Additional DAZL Products

Product Documents for DAZL Antibody (DAZL/4253)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DAZL Antibody (DAZL/4253)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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