DBF4 Antibody (6G9)

Novus Biologicals, part of Bio-Techne | Catalog # H00010926-M01

Novus Biologicals, part of Bio-Techne
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H00010926-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6G9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DBF4 Antibodies »

Product Specifications

Immunogen

DBF4 (NP_006707, 2 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQT

Reactivity Notes

Human. Other species not tested.

Specificity

DBF4 - DBF4 homolog (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for DBF4 Antibody (6G9)

Western Blot: DBF4 Antibody (6G9) [H00010926-M01]

Western Blot: DBF4 Antibody (6G9) [H00010926-M01]

Western Blot: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.Lane 1: DBF4 transfected lysate(76.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: DBF4 Antibody (6G9) [H00010926-M01]

Immunocytochemistry/ Immunofluorescence: DBF4 Antibody (6G9) [H00010926-M01]

Immunocytochemistry/Immunofluorescence: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of monoclonal antibody to DBF4 on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: DBF4 Antibody (6G9) [H00010926-M01]

Immunohistochemistry-Paraffin: DBF4 Antibody (6G9) [H00010926-M01]

Immunohistochemistry-Paraffin: DBF4 Antibody (6G9) [H00010926-M01] - Immunoperoxidase of monoclonal antibody to DBF4 ( Cat # H00010926-M01 ) on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
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Applications for DBF4 Antibody (6G9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DBF4

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle

Alternate Names

ASKchif, DBF4 homolog (S. cerevisiae), DBF4ACHIF, DBF4-type zinc finger-containing protein 1, ZDBF1DBF-type containing 1

Entrez Gene IDs

10926 (Human)

Gene Symbol

DBF4

OMIM

604281 (Human)

UniProt

NP_006707 (Human)

Additional DBF4 Products

Product Documents for DBF4 Antibody (6G9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DBF4 Antibody (6G9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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