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DBPA Antibody (4D9)

Novus Biologicals, part of Bio-Techne | Catalog # H00008531-M02

Novus Biologicals, part of Bio-Techne
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H00008531-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4D9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CSDA (NP_003642, 241 a.a. ~ 330 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HVGQTFDRRSRVLPHPNRIQAGEIGEMKDGVPEGAQLQGPVHRNPTYRPRYRSRGPPRPRPAPAVGEAEDKENQQATSGPNQPSVRRGYR

Specificity

CSDA - cold shock domain protein A

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DBPA Antibody (4D9)

Western Blot: DBPA Antibody (4D9) [H00008531-M02]

Western Blot: DBPA Antibody (4D9) [H00008531-M02]

Western Blot: DBPA Antibody (4D9) [H00008531-M02] - Analysis of CSDA expression in transfected 293T cell line by CSDA monoclonal antibody (M02), clone 4D9.Lane 1: CSDA transfected lysate(40.1 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: DBPA Antibody (4D9) [H00008531-M02]

Immunohistochemistry-Paraffin: DBPA Antibody (4D9) [H00008531-M02]

Immunohistochemistry-Paraffin: DBPA Antibody (4D9) [H00008531-M02] - Analysis of monoclonal antibody to CSDA on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Sandwich ELISA: DBPA Antibody (4D9) [H00008531-M02] - Detection limit for recombinant GST tagged CSDA is approximately 0.3ng/ml as a capture antibody.

Applications for DBPA Antibody (4D9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DBPA

DBPA binds to the GM-CSF promoter. Seems to act as a repressor. Binds also to full length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3'. May have a role in translation repression

Alternate Names

cold shock domain protein A, Cold shock domain-containing protein A, cold-shock domain protein A, CSDA1, dbpA, DBPAcold-shock domain containing A1, DNA-binding protein A, Single-strand DNA-binding protein NF-GMB, ZO-1-associated nucleic acid-binding protein, ZONAB

Entrez Gene IDs

8531 (Human)

Gene Symbol

YBX3

OMIM

603437 (Human)

Additional DBPA Products

Product Documents for DBPA Antibody (4D9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DBPA Antibody (4D9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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