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DBPA Antibody (PCRP-YBX3-2D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23901

Novus Biologicals, part of Bio-Techne
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NBP3-23901-100ug
NBP3-23901-20ug

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-YBX3-2D12

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant full-length human DBPA protein

Localization

Nucleus. Cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for DBPA Antibody (PCRP-YBX3-2D12)

DBPA Antibody (PCRP-YBX3-2D12)

Protein Array: DBPA Antibody (PCRP-YBX3-2D12) [NBP3-23901] -

Analysis of Protein Array containing more than 19,000 full-length human proteins using DBPA antibody (PCRP-YBX3-2D12)
DBPA Antibody (PCRP-YBX3-2D12)

Immunohistochemistry-Paraffin: DBPA Antibody (PCRP-YBX3-2D12) [NBP3-23901] -

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with DBPA antibody (PCRP-YBX3-2D12). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
DBPA Antibody (PCRP-YBX3-2D12)

Flow Cytometry: DBPA Antibody (PCRP-YBX3-2D12) [NBP3-23901] -

Flow cytometric analysis of PFA-fixed HeLa cells. DBPA antibody (PCRP-YBX3-2D12) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Applications for DBPA Antibody (PCRP-YBX3-2D12)

Application
Recommended Usage

Flow Cytometry

1-2ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

1-2ug/ml

Immunoprecipitation

1-2ug/100-500ug protein

Western Blot

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: DBPA

DBPA binds to the GM-CSF promoter. Seems to act as a repressor. Binds also to full length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3'. May have a role in translation repression

Alternate Names

cold shock domain protein A, Cold shock domain-containing protein A, cold-shock domain protein A, CSDA1, dbpA, DBPAcold-shock domain containing A1, DNA-binding protein A, Single-strand DNA-binding protein NF-GMB, ZO-1-associated nucleic acid-binding protein, ZONAB

Gene Symbol

YBX3

Additional DBPA Products

Product Documents for DBPA Antibody (PCRP-YBX3-2D12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DBPA Antibody (PCRP-YBX3-2D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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