Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DBPA Antibodies »

Product Summary for DBPA Antibody

Immunogen

The immunogen for this product maps to a region between residue 250 and 300 of human ZO-1-associated nucleic acid-binding protein using the numbering given in entry NP_003642.3 (GeneID 8531).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DBPA Antibody

Western Blot: DBPA Antibody [NBP1-71827]

Western Blot: DBPA Antibody [NBP1-71827]

Western Blot: DBPA Antibody [NBP1-71827] - Whole cell lysate (50 ug) from MCF-7, HeLa, Hep-G2, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZONABantibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: DBPA Antibody [NBP1-71827]

Immunoprecipitation: DBPA Antibody [NBP1-71827]

Immunoprecipitation: DBPA Antibody [NBP1-71827] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZONAB antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ZONAB was also immunoprecipitated by another rabbit anti-ZONAB antibody which recognizes a downstream epitope.

Applications for DBPA Antibody

Application
Recommended Usage

Immunoprecipitation

2- 10 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DBPA

This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18.5 kDa in size corresponding to Borrelia burgdorferi DbpA protein by Western blotting in the appropriate cell lysate or extract.

Alternate Names

cold shock domain protein A, Cold shock domain-containing protein A, cold-shock domain protein A, CSDA1, dbpA, DBPAcold-shock domain containing A1, DNA-binding protein A, Single-strand DNA-binding protein NF-GMB, ZO-1-associated nucleic acid-binding protein, ZONAB

Gene Symbol

YBX3

UniProt

NP_003642.3 (Human)

Additional DBPA Products

Product Documents for DBPA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DBPA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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