DC-SIGN/CD209 Antibody (LWC06) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43328

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Flow Cytometry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # LWC06

Format

BSA Free

Concentration

0.5 mg/ml

View all DC-SIGN/CD209 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CIRE.

Marker

Dendritic Cell Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for DC-SIGN/CD209 Antibody (LWC06) - BSA Free

Western Blot: DC-SIGN/CD209 Antibody (LWC06)BSA Free [NBP1-43328]

Western Blot: DC-SIGN/CD209 Antibody (LWC06)BSA Free [NBP1-43328]

Western Blot: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - CIRE/DC-SIGN-transfected (left) and untransfected control CHO lysates were loaded at 1x10^5 cells/lane, probed with 1 ug/mL of Anti-Mouse CD209 (DC-SIGN) Purified and revealed with Anti-Rat IgG HRP.
Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) - BSA Free [NBP1-43328]

Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) - BSA Free [NBP1-43328]

Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the PE conjugate of NBP1-43328..
Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) - BSA Free [NBP1-43328]

Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) - BSA Free [NBP1-43328]

Flow Cytometry: DC-SIGN/CD209 Antibody (LWC06) [NBP1-43328] - Analysis using the Allophycocyanin conjugate of NBP1-43328. Staining of mouse CIRE/DC-SIGN-transfected CHO cells with 0.125 ug of Rat IgG2a kappa Isotype Control APC (blue histogram) or 0.125 ug of Anti-Mouse CD209 (DC-SIGN) APC (purple histogram).
View 1 more images

Applications for DC-SIGN/CD209 Antibody (LWC06) - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This LWC06 antibody has been tested by immunoblotting and immunoprecipitation of CIRE in CIRE-transfected CHO cells. The reactivity of this antibody has been confirmed by immunoprecipitation of CIRE with LWC06 followed by immunoblotting with another CIRE-specific monoclonal antibody, 5H10 and, immunoprecipitation of CIRE with 5H10 followed by immunoblotting with LWC06.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DC-SIGN/CD209

The LWC06 antibody was generated by immunization with the recombinant extracellular region of mouse CIRE/DC-SIGN (CD209). CIRE/DC-SIGN was identified by its expression on CD8alpha- dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN. Human DC-SIGN was originally identified in human placenta for its ability to bind the HIV envelope protein gp120 in a CD4-independent manner. CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein. It contains a C-terminal, extracellular, Carbohydrate Recognition Domain (CRD) that is predicted to bind mannose and other carbohydrates in a Ca2+ dependent manner. It has been postulated that CIRE/DC-SIGN may play a role in T-dendritic cell interactions through binding with members of the ICAM family. CIRE/DC-SIGN is differentially expressed by sub-populations of dendritic cells and preliminary data suggest that its expression varies depending on the activation state of the host. CIRE/DC-SIGN is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. While human DC-SIGN is predominantly expressed in dendritic cells, CIRE/DC-SIGN mRNA has also been detected in B cells. The LWC06 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1

Alternate Names

CD209, CLEC4L, DC-SIGN1, DCSIGN

Gene Symbol

CD209

Additional DC-SIGN/CD209 Products

Product Documents for DC-SIGN/CD209 Antibody (LWC06) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DC-SIGN/CD209 Antibody (LWC06) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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