DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52649

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 16E7

Format

Azide and BSA Free

Concentration

1 mg/ml

View all DC-SIGNR/CD299 Antibodies »

Product Summary for DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free

Immunogen

CD299

Specificity

Human CD299, which is an oligomeric type II transmembrane protein with a C-type lectin extracellular domain, the expression of which is restricted to immature DC, macrophages in the lung, and endothelial cells in the liver. It binds ICAM-3 and ICAM-7 to mediate the interaction of DC with T lymphocytes and endothelial cells in the initial stages of immune response and in the migratory behavior of DC. CD299 also binds the gp120 protein of HIV and the E2 envelope protein of HCV, thereby playing a role in viral infection.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free

Western Blot: DC-SIGNR/CD299/CLEC4M Antibody (16E7)ChimericAzide and BSA Free [NBP2-52649]

Western Blot: DC-SIGNR/CD299/CLEC4M Antibody (16E7)ChimericAzide and BSA Free [NBP2-52649]

Western Blot: DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric [NBP2-52649] - Western Blot using anti-DC-SIGNR (CLEC4M) antibody 16E7. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 16E7 (NBP2-52649) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for DC-SIGNR is 45.3kDa, though 9 other isoforms of this protein are known ranging in size from 22.4-44.7kDa (Uniprot ID: Q9H2X3). DC-SIGNR is also glycosylated at several positions. NBP2-52649 successfully detected the canonical human DC-SIGNR, as well as multiple other isoforms.
Immunocytochemistry/ Immunofluorescence: DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free [NBP2-52649]

Immunocytochemistry/ Immunofluorescence: DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free [NBP2-52649]

Immunocytochemistry/Immunofluorescence: DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric [NBP2-52649] - Immunofluorescence: [NBP2-52649] - Immunofluoresence staining of fixed HeLa cells with anti-DC-SIGNR antibody 16E7. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of 16E7 (NBP2-52649) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52649, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

Applications for DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: DC-SIGNR/CD299

Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD209) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN, CD209L or CD299) are type II transmembrane proteins that are mannose-specific, Calcium-dependent (C-type) lectins.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 2

Alternate Names

CD209L, CD299, CLEC4M, DC-SIGN2, DCSIGNR, LSIGN

Gene Symbol

CLEC4M

Additional DC-SIGNR/CD299 Products

Product Documents for DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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