DCAF11 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-57691
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Synthetic peptides corresponding to WDR23(WD repeat domain 23) The peptide sequence was selected from the N terminal of WDR23. Peptide sequence GSRNSSSAGSGSGDPSEGLPRRGAGLRRSEEEEEEDEDVDLAQVLAYLLR. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for DCAF11 Antibody
![Western Blot: DCAF11 Antibody [NBP1-57691]](https://resources.bio-techne.com/images/products/DCAF11-Antibody-Western-Blot-NBP1-57691-img0006.jpg "Western Blot: DCAF11 Antibody [NBP1-57691]")
Western Blot: DCAF11 Antibody [NBP1-57691]
Western Blot: DCAF11 Antibody [NBP1-57691] - TFEB represses the expression of DCAF11. The protein levels of Keap1 and Nrf2-specific E3 ubiquitin ligases in H4 and TFEB cells were analyzed by immunoblotting using each corresponding antibody. Full blots are provided in Supplementary Fig. S10. Data shown are mean +/- S.E. of three independent experiments and were analyzed using Student's t test. (**p < 0.01; ***p < 0.001). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-019-50877-8) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: DCAF11 Antibody [NBP1-57691] -
Immunohistochemistry-Paraffin: DCAF11 Antibody [NBP1-57691] - Formalin Fixed Paraffin; Embedded Tissue: Human Adult Liver; Observed Staining: Cytoplasm in hepatocytes, strong signal, moderate tissue distribution; Primary Antibody Concentration: 1:100![Western Blot: DCAF11 Antibody [NBP1-57691]](https://resources.bio-techne.com/images/products/DCAF11-Antibody-Western-Blot-NBP1-57691-img0003.jpg "Western Blot: DCAF11 Antibody [NBP1-57691]")
Western Blot: DCAF11 Antibody [NBP1-57691]
Western Blot: DCAF11 Antibody [NBP1-57691] - Titration: 0.2-1 ug/ml, Positive Control: Human Placenta.Applications for DCAF11 Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DCAF11
Alternate Names
DDB1 and CUL4 associated factor 11, DDB1- and CUL4-associated factor 11, GL014, PRO2389, WD repeat domain 23, WD repeat-containing protein 23, WDR23DKFZp779A1629
Gene Symbol
DCAF11
UniProt
Additional DCAF11 Products
Product Documents for DCAF11 Antibody
Product Specific Notices for DCAF11 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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