DCAF4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05972
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human WD repeat-containing protein 21A using the numbering given in entry NP_056419.2 (GeneID 26094).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DCAF4 Antibody
![Western Blot: DCAF4 Antibody [NBP1-05972]](https://resources.bio-techne.com/images/products/DCAF4-Antibody-Western-Blot-NBP1-05972-img0003.jpg "Western Blot: DCAF4 Antibody [NBP1-05972]")
Western Blot: DCAF4 Antibody [NBP1-05972]
Western Blot: DCAF4 Antibody [NBP1-05972] - Detection of Human and Mouse WDR21A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WDR21A antibody NBP1-05972 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.![Immunoprecipitation: DCAF4 Antibody [NBP1-05972]](https://resources.bio-techne.com/images/products/DCAF4-Antibody-Immunoprecipitation-NBP1-05972-img0004.jpg "Immunoprecipitation: DCAF4 Antibody [NBP1-05972]")
Immunoprecipitation: DCAF4 Antibody [NBP1-05972]
Immunoprecipitation: DCAF4 Antibody [NBP1-05972] - Detection of human WDR21A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WDR21A antibody NBP1-05972 (lot NBP1-05972-2) used for IP at 6 ug per reaction. WDR21A was also immunoprecipitated by a previous lot of this antibody (lot NBP1-05972-1) and another rabbit anti-WDR21A antibody. For blotting immunoprecipitated WDR21A, NBP1-05972 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for DCAF4 Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 5 µg/mg lysate
Western Blot
1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DCAF4
Alternate Names
DDB1 and CUL4 associated factor 4, DDB1- and CUL4-associated factor 4, DKFZp434K114, MGC20547, MGC46524, WD repeat domain 21, WD repeat domain 21A, WD repeat-containing protein 21A, WDR21, WDR21A
Entrez Gene IDs
26094 (Human)
Gene Symbol
DCAF4
UniProt
Additional DCAF4 Products
Product Documents for DCAF4 Antibody
Product Specific Notices for DCAF4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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