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DCI Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91822

Novus Biologicals, part of Bio-Techne
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NBP1-91822
NBP1-91822-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FGSQRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTELVISLEKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DCI Antibody

Immunohistochemistry-Paraffin: DCI Antibody [NBP1-91822]

Immunohistochemistry-Paraffin: DCI Antibody [NBP1-91822]

Immunohistochemistry-Paraffin: DCI Antibody [NBP1-91822] - Analysis in human duodenum and tonsil tissues using NBP1-91822 antibody. Corresponding ECI1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: DCI Antibody [NBP1-91822]

Immunohistochemistry-Paraffin: DCI Antibody [NBP1-91822]

Immunohistochemistry-Paraffin: DCI Antibody [NBP1-91822] - Staining of human cerebral cortex, kidney, liver and testis using Anti-ECI1 antibody NBP1-91822 (A) shows similar protein distribution across tissues to independent antibody NBP1-91821 (B).
Western Blot: DCI Antibody [NBP1-91822]

Western Blot: DCI Antibody [NBP1-91822]

Western Blot: DCI Antibody [NBP1-91822] - Analysis using Anti-ECI1 antibody NBP1-91822 (A) shows similar pattern to independent antibody NBP1-91821 (B).

Applications for DCI Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DCI

The DCI gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising durin

Alternate Names

acetylene-allene isomerase, D2-enoyl-CoA isomerase, D3, DCI3,2-trans-enoyl-CoA isomerase3,2 trans-enoyl-CoA isomerase, Delta(2)-enoyl-CoA isomerase, Delta(3), delta3, delta2-enoyl-CoA isomerase, dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase), dodecenoyl-CoA isomerase3,2-trans-enoyl-CoA isomerase, mitochondrial, dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase), EC 5.3.3.8, enoyl-CoA delta isomerase 13,2 trans-enoyl-Coenzyme A isomerase

Gene Symbol

ECI1

Additional DCI Products

Product Documents for DCI Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DCI Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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