DCIR/CLEC4A Antibody (9E8) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: DCIR/CLEC4A Antibody (9E8) - Chimeric - Azide and BSA Free [NBP3-09023]

Immunocytochemistry/Immunofluorescence: DCIR/CLEC4A Antibody (9E8) - Chimeric [NBP3-09023] - Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of 9E8 (NBP3-09023) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09023, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: DCIR/CLEC4A Antibody (9E8) - Chimeric - Azide and BSA Free [NBP3-09023]

Flow Cytometry: DCIR/CLEC4A Antibody (9E8) - Chimeric [NBP3-09023] - Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of 9E8 (NBP3-09023, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.