DCK Antibody (OTI3F5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70555

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3F5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DCK Antibodies »

Product Summary for DCK Antibody (OTI3F5) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human DCK (NP_000779) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

30.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DCK Antibody (OTI3F5) - Azide and BSA Free

Western Blot: DCK Antibody (OTI3F5)Azide and BSA Free [NBP2-70555]

Western Blot: DCK Antibody (OTI3F5)Azide and BSA Free [NBP2-70555]

Western Blot: DCK Antibody (OTI3F5) - Azide and BSA Free [NBP2-70555] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCK.
Immunocytochemistry/ Immunofluorescence: DCK Antibody (OTI3F5) - Azide and BSA Free [NBP2-70555]

Immunocytochemistry/ Immunofluorescence: DCK Antibody (OTI3F5) - Azide and BSA Free [NBP2-70555]

Immunocytochemistry/Immunofluorescence: DCK Antibody (OTI3F5) - Azide and BSA Free [NBP2-70555] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DCK.
Immunohistochemistry: DCK Antibody (OTI3F5) - Azide and BSA Free [NBP2-70555]

Immunohistochemistry: DCK Antibody (OTI3F5) - Azide and BSA Free [NBP2-70555]

Immunohistochemistry: DCK Antibody (OTI3F5) - Azide and BSA Free [NBP2-70555] - Staining of paraffin-embedded Human lymph node tissue using anti-DCK mouse monoclonal antibody.
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Applications for DCK Antibody (OTI3F5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DCK

DCK (Deoxycytidine kinase) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.

Long Name

Deoxycytidine kinase

Alternate Names

EC 2.7.1.113, EC 2.7.1.74, EC 2.7.1.76

Gene Symbol

DCK

Additional DCK Products

Product Documents for DCK Antibody (OTI3F5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DCK Antibody (OTI3F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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