Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DCLK1 Antibodies »

Product Summary for DCLK1 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 690 to 740 of human Doublecortin-Like and CAM Kinase-Like 1 using the numbering given in entry O15075.2 (GeneID 9201).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DCLK1 Antibody

Western Blot: DCLK1 Antibody [NBP2-36420]

Western Blot: DCLK1 Antibody [NBP2-36420]

Western Blot: DCLK1 Antibody [NBP2-36420] - Detection of Human and Mouse DCAMKL1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-DCAMKL1 antibody NBP2-36420 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: DCLK1 Antibody [NBP2-36420]

Immunoprecipitation: DCLK1 Antibody [NBP2-36420]

Immunoprecipitation: DCLK1 Antibody [NBP2-36420] - Detection of human DCAMKL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T and cells. Antibodies: Affinity purified rabbit anti-DCAMKL1 antibody NBP2-36420 used for IP at 6 ug per reaction. DCAMKL1 was also immunoprecipitated by another rabbit anti-DCAMKL1 antibody. For blotting immunoprecipitated DCAMKL1, NBP2-36420 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for DCLK1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:500 - 1:2500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DCLK1

DCAMKL1 is a microtubule-associated protein that phosphorylates itself and myelin basic protein (MBP; MIM 159430). DCAMKL1 has microtubule polymerizing activity that is independent of its protein kinase activity (Lin et al., 2000 [PubMed 11124993]).[supplied by OMIM]

Long Name

Doublecortin-like and CAM Kinase-like 1

Alternate Names

CL1, CLICK1, DCAMKL1, DCDC3A

Gene Symbol

DCLK1

UniProt

O15075.2 (Human)

Additional DCLK1 Products

Product Documents for DCLK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DCLK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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