Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DCLK1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 690 to 740 of human Doublecortin-Like and CAM Kinase-Like 1 using the numbering given in entry O15075.2 (GeneID 9201).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DCLK1 Antibody

Western Blot: DCLK1 Antibody [NBP2-36420]

Western Blot: DCLK1 Antibody [NBP2-36420]

Western Blot: DCLK1 Antibody [NBP2-36420] - Detection of Human and Mouse DCAMKL1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-DCAMKL1 antibody NBP2-36420 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: DCLK1 Antibody [NBP2-36420]

Immunoprecipitation: DCLK1 Antibody [NBP2-36420]

Immunoprecipitation: DCLK1 Antibody [NBP2-36420] - Detection of human DCAMKL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T and cells. Antibodies: Affinity purified rabbit anti-DCAMKL1 antibody NBP2-36420 used for IP at 6 ug per reaction. DCAMKL1 was also immunoprecipitated by another rabbit anti-DCAMKL1 antibody. For blotting immunoprecipitated DCAMKL1, NBP2-36420 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for DCLK1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:500 - 1:2500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DCLK1

DCAMKL1 is a microtubule-associated protein that phosphorylates itself and myelin basic protein (MBP; MIM 159430). DCAMKL1 has microtubule polymerizing activity that is independent of its protein kinase activity (Lin et al., 2000 [PubMed 11124993]).[supplied by OMIM]

Long Name

Doublecortin-like and CAM Kinase-like 1

Alternate Names

CL1, CLICK1, DCAMKL1, DCDC3A

Gene Symbol

DCLK1

UniProt

O15075.2 (Human)

Additional DCLK1 Products

Product Documents for DCLK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DCLK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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