Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Dcp1a Antibodies »

Product Summary for Dcp1a Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 225 and 275 of human DCP1 Decapping Enzyme Homolog A using the numbering given in entry NP_060873.4 (GeneID 55802).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dcp1a Antibody

Immunoprecipitation: Dcp1a Antibody [NBP2-04038]

Immunoprecipitation: Dcp1a Antibody [NBP2-04038]

Immunoprecipitation: Dcp1a Antibody [NBP2-04038] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DCP1A antibody NBP2-04013 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. DCP1A was also immunoprecipitated by rabbit anti-DCP1A antibodies NBP2-04038 and NBP2-04039, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Applications for Dcp1a Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Dcp1a

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway.

Alternate Names

DCP1 decapping enzyme homolog A (S. cerevisiae), decapping enzyme hDcp1a, EC 3.-, FLJ21691, mRNA-decapping enzyme 1A, Nbla00360, putative protein product of Nbla00360, Smad4-interacting transcriptional co-activator, SMAD4IP1, SMIFHSA275986, Transcription factor SMIF

Gene Symbol

DCP1A

UniProt

NP_060873.4 (Human)

Additional Dcp1a Products

Product Documents for Dcp1a Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Dcp1a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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