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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 370 and 420 of human mRNA-decapping enzyme 2 using the numbering given in entry NP_689837.2 (GeneID 167227).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DCP2 Antibody - BSA Free

Western Blot: DCP2 Antibody [NBP1-41070]

Western Blot: DCP2 Antibody [NBP1-41070]

Western Blot: DCP2 Antibody [NBP1-41070] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Affinity purified rabbit anti-Dcp2 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. Dcp2 was also immunoprecipitated by another rabbit anti-Dcp2 antibody, which recognizes an upstream epitope.

Applications for DCP2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 27918561)

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DCP2

DCP2 is a key component of an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end (Fenger-Gron et al., 2005 [PubMed 16364915]).[supplied by OMIM]

Alternate Names

DCP2 decapping enzyme homolog (S. cerevisiae), EC 3.-, FLJ33245, hDpc, mRNA-decapping enzyme 2, nudix (nucleoside diphosphate linked moiety X)-type motif 20, Nudix motif 20, NUDT20Nucleoside diphosphate-linked moiety X motif 20

Entrez Gene IDs

167227 (Human)

Gene Symbol

DCP2

Additional DCP2 Products

Product Documents for DCP2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DCP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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