DCP2 Antibody

Western Blot: DCP2 Antibody [NBP2-16109] -

Western Blot: DCP2 Antibody [NBP2-16109] -A. 30 μg 24hr whole zebrafish lysate/extract10 % SDS-PAGEDCP2 antibody dilution: 1:500

Western Blot: DCP2 Antibody [NBP2-16109] -

Western Blot: DCP2 Antibody [NBP2-16109] -Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DCP2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: DCP2 Antibody [NBP2-16109] -

Immunocytochemistry/ Immunofluorescence: DCP2 Antibody [NBP2-16109] - rbpms2 mutant allele stability & localization activity in somatic cells.(A) RT-PCR to detect rbpms2a & rbpms2b maternal transcripts. Pink asterisks indicate likely nonsense mediated decay of mutant allele transcripts. (B-F) Wild-type GFP-Rbpms2a/b (B, D) & GFP-Rbpms2bsa9329 (F) localize to granules in HEK 293 cells, while GFP-Rbpms2aae30 & GFP-Rbpms2bae32 are not localized. (G-K) Zebrafish blastula cells expressing GFP-Rbpms2 fusions. (G,H) Wild-type GFP-Rbpms2b localizes near the nucleus (H), is apparently associated with the centrosome/spindle in some cells, & (G) is in granules that are not positive for the stress granule marker Tial-1. (H) A subset of GFP-Rbpms2b positive granules are positive for the p-body marker Dcp2 (open arrowheads). (I) GFP-Rbpms2bsa9329 localization to the centrosome/spindle but not granules. (J) GFP-Rbpms2bae32 & (K) GFP-Rbpms2aae30 localization. Insets show magnified views of the highlighted cells. Images are representative slices from Z-stacks of sphere stage embryos viewed from the animal pole. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29975683), licensed under a CC-BY license. Not internally tested by Novus Biologicals.