DCTN3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00011258-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DCTN3 Antibodies »

Product Specifications

Immunogen

DCTN3 (NP_077324.1, 1 a.a. - 176 a.a.) full-length human protein. MAGLTDLQRLQARVEELERWVYGPGGARGSRKVADGLVKVQVALGNISSKRERVKILYKKIEDLIKYLDPEYIDRIAIPDASKLQFILAEEQFILSQVALLEQVNALVPMLDSAHIKAVPEHAARLQRLAQIHIQQQAPWGVGVRDEAGSLVEDVGFAQFLSVLHFGPTGPVCGNH

Specificity

DCTN3 - dynactin 3 (p22),

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for DCTN3 Antibody

Western Blot: DCTN3 Antibody [H00011258-B01P]

Western Blot: DCTN3 Antibody [H00011258-B01P]

Western Blot: DCTN3 Antibody [H00011258-B01P] - Analysis of DCTN3 expression in transfected 293T cell line by DCTN3 polyclonal antibody. Lane 1: DCTN3 transfected lysate(19.36 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: DCTN3 Antibody [H00011258-B01P]

Immunocytochemistry/ Immunofluorescence: DCTN3 Antibody [H00011258-B01P]

Immunocytochemistry/Immunofluorescence: DCTN3 Antibody [H00011258-B01P] - Analysis of purified antibody to DCTN3 on HeLa cell. (antibody concentration 10 ug/ml)

Applications for DCTN3 Antibody

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. Has also been used for immunofluorescence.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DCTN3

This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Alternate Names

DCTN22DCTN-22, dynactin 3, dynactin 3 (p22), Dynactin complex subunit 22 kDa subunit, dynactin light chain, dynactin subunit 3, MGC111190, p22

Entrez Gene IDs

11258 (Human)

Gene Symbol

DCTN3

UniProt

NP_077324.1 (Human)

Additional DCTN3 Products

Product Documents for DCTN3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DCTN3 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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