DCTN3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52105
Key Product Details
Species Reactivity
Validated:
Human, Rat
Predicted:
Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-QVALGNISSKRE corresponding to internal region according to NP_009165.1; NP_077324.1.
Epitope
C-QVALGNISSKRE
Specificity
This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for DCTN3 Antibody
Western Blot: DCTN3 Antibody [NBP1-52105]
Western Blot: DCTN3 Antibody [NBP1-52105] - Staining (0.03ug/ml) of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: DCTN3 Antibody [NBP1-52105]
Immunohistochemistry-Paraffin: DCTN3 Antibody [NBP1-52105] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.Flow Cytometry: DCTN3 Antibody [NBP1-52105]
Flow Cytometry: DCTN3 Antibody [NBP1-52105] - Analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for DCTN3 Antibody
Application
Recommended Usage
Flow Cytometry
10ug/ml
Immunohistochemistry-Paraffin
6 - 8 ug/ml
Peptide ELISA
Detection limit 1:16000
Western Blot
0.1 - 0.3 ug/ml
Application Notes
WB: Approx 20 kDa band observed in Human Liver lysates, and approx. 18 kDa in Rat Kidney lysates and in preliminary testing of Mouse Kidney lysate (calculated MW of 19. 5kDa according to NP_077324.1 and 21.1 kDa according to NP_001102129.1.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DCTN3
Alternate Names
DCTN22DCTN-22, dynactin 3, dynactin 3 (p22), Dynactin complex subunit 22 kDa subunit, dynactin light chain, dynactin subunit 3, MGC111190, p22
Gene Symbol
DCTN3
UniProt
Additional DCTN3 Products
Product Documents for DCTN3 Antibody
Product Specific Notices for DCTN3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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