DCUN1D4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81526

Novus Biologicals, part of Bio-Techne
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NBP1-81526-25ul
NBP1-81526
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DCUN1D4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FNKVMPPRKKRRPASGDDLSAKKSRHDSMYRKYDSTRIKTEEEAFSSKRCLEWFYE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DCUN1D4 Antibody

Western Blot: DCUN1D4 Antibody [NBP1-81526]

Western Blot: DCUN1D4 Antibody [NBP1-81526]

Western Blot: DCUN1D4 Antibody [NBP1-81526] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: DCUN1D4 Antibody [NBP1-81526]

Immunocytochemistry/ Immunofluorescence: DCUN1D4 Antibody [NBP1-81526]

Immunocytochemistry/Immunofluorescence: DCUN1D4 Antibody [NBP1-81526] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & vesicles.
Immunohistochemistry-Paraffin: DCUN1D4 Antibody [NBP1-81526]

Immunohistochemistry-Paraffin: DCUN1D4 Antibody [NBP1-81526]

Immunohistochemistry-Paraffin: DCUN1D4 Antibody [NBP1-81526] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.
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Applications for DCUN1D4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DCUN1D4

Alternate Names

DCN1, defective in cullin neddylation 1, domain containing 4 (S. cerevisiae), DCN1-like protein 4, DCUN1 domain-containing protein 4, FLJ42355, KIAA0276Defective in cullin neddylation protein 1-like protein 4

Gene Symbol

DCUN1D4

Additional DCUN1D4 Products

Product Documents for DCUN1D4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DCUN1D4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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