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DDAH2 Antibody (5M4Y4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16345

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16345-100ul
NBP3-16345-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5M4Y4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for DDAH2 Antibody (5M4Y4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DDAH2 (O95865). MGTPGEGLGRCSHALIRGVPESLASGEGAGAGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLPLGPLLGDTAVIQGDTALITRPWSPARRPEV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDAH2 Antibody (5M4Y4)

Western Blot: DDAH2 Antibody (5M4Y4) [NBP3-16345]

Western Blot: DDAH2 Antibody (5M4Y4) [NBP3-16345]

Western Blot: DDAH2 Antibody (5M4Y4) [NBP3-16345] - Western blot analysis of extracts of various cell lines, using DDAH2 Rabbit mAb (NBP3-16345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for DDAH2 Antibody (5M4Y4)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDAH2

DDAH2 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Overexpression of DDAH2 has also been linked to increased VEGF mRNA expression and enhanced tube formation by cells grown in a 3-dimensional medium.

Alternate Names

DDAHII, dimethylargininase-2, dimethylarginine dimethylaminohydrolase 2G6a, dimethylarginine dimethylaminohydrolase II, EC 3.5.3.18, G6a, N(G), N(G)-dimethylarginine dimethylaminohydrolase 2, NG30, NG-dimethylarginine dimethylamino hydrolase homolog, S-phase protein

Gene Symbol

DDAH2

Additional DDAH2 Products

Product Documents for DDAH2 Antibody (5M4Y4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DDAH2 Antibody (5M4Y4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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