DDB1 Antibody (C.15200221)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62791

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # C.15200221

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DDB1 Antibodies »

Product Specifications

Immunogen

Human DDB1 (Damage-Specific DNA Binding Protein 1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for DDB1 Antibody (C.15200221)

Western Blot: DDB1 Antibody (C.15200221) [NBP2-62791]

Western Blot: DDB1 Antibody (C.15200221) [NBP2-62791]

Western Blot: DDB1 Antibody (C.15200221) [NBP2-62791] - Whole cell extracts from human HeLa cells were analyzed using the antibody against DDB1 diluted 1:500 in PBS containing 10% milk. The position of the protein of interest (expected MW 127 kDa) is indicated on the right; the marker (in kDa) is shown on the left.

Applications for DDB1 Antibody (C.15200221)

Application
Recommended Usage

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDB1

Damage specific DNA binding protein (DDB) functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, purified human DDB protein was injected into XPE cells. The injected DDB protein stimulated DNA repair to normal levels in those strains that lacked the DDB activity but did not stimulate repair in cells from XPE patients that contained the activity. These results provided direct evidence that defective DDB activity causes the repair defect in a subset of XPE patients and establishes a role for this activity in nucleotide excision repair in vivo. It remains for mutation analysis to demonstrate whether the defect in XPE patients is in the DDB1 gene or the DDB2 gene.

Long Name

Damage-specific DNA Binding Protein 1

Alternate Names

DDB p127 Subunit, DDBA, UV-DDB 1, XPCE

Gene Symbol

DDB1

Additional DDB1 Products

Product Documents for DDB1 Antibody (C.15200221)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DDB1 Antibody (C.15200221)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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