DDB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98785

Novus Biologicals, part of Bio-Techne
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NBP2-98785-100ul
NBP2-98785-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DDB1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human DDB1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for DDB1 Antibody

Immunocytochemistry/ Immunofluorescence: DDB1 Antibody [NBP2-98785]

Immunocytochemistry/ Immunofluorescence: DDB1 Antibody [NBP2-98785]

Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP2-98785] - Staining of DDB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DDB1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Immunohistochemistry-Paraffin: DDB1 Antibody [NBP2-98785]

Immunohistochemistry-Paraffin: DDB1 Antibody [NBP2-98785]

Immunohistochemistry-Paraffin: DDB1 Antibody [NBP2-98785] - Staining of human DDB1 in human breast with rabbit polyclonal antibody (1:1000).

Applications for DDB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDB1

Damage specific DNA binding protein (DDB) functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, purified human DDB protein was injected into XPE cells. The injected DDB protein stimulated DNA repair to normal levels in those strains that lacked the DDB activity but did not stimulate repair in cells from XPE patients that contained the activity. These results provided direct evidence that defective DDB activity causes the repair defect in a subset of XPE patients and establishes a role for this activity in nucleotide excision repair in vivo. It remains for mutation analysis to demonstrate whether the defect in XPE patients is in the DDB1 gene or the DDB2 gene.

Long Name

Damage-specific DNA Binding Protein 1

Alternate Names

DDB p127 Subunit, DDBA, UV-DDB 1, XPCE

Gene Symbol

DDB1

Additional DDB1 Products

Product Documents for DDB1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DDB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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