DDHD2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82965
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for DDHD2 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RMHLELREGLTRMSMDLKNNLLGSLRMAWKSFTRAPYPALQASETPEETEAEPESTSEKPSDVNTEETSVAVKEEVLPINVGMLNGGQRIDYVLQEK
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (85%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DDHD2 Antibody
![Western Blot: DDHD2 Antibody [NBP1-82965]](https://resources.bio-techne.com/images/products/DDHD2-Antibody-Western-Blot-NBP1-82965-img0006.jpg "Western Blot: DDHD2 Antibody [NBP1-82965]")
Western Blot: DDHD2 Antibody [NBP1-82965]
Western Blot: DDHD2 Antibody [NBP1-82965] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue![Immunocytochemistry/ Immunofluorescence: DDHD2 Antibody [NBP1-82965]](https://resources.bio-techne.com/images/products/DDHD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82965-img0009.jpg "Immunocytochemistry/ Immunofluorescence: DDHD2 Antibody [NBP1-82965]")
Immunocytochemistry/ Immunofluorescence: DDHD2 Antibody [NBP1-82965]
Immunocytochemistry/Immunofluorescence: DDHD2 Antibody [NBP1-82965] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.![Immunohistochemistry-Paraffin: DDHD2 Antibody [NBP1-82965]](https://resources.bio-techne.com/images/products/DDHD2-Antibody-Immunohistochemistry-Paraffin-NBP1-82965-img0004.jpg "Immunohistochemistry-Paraffin: DDHD2 Antibody [NBP1-82965]")
Immunohistochemistry-Paraffin: DDHD2 Antibody [NBP1-82965]
Immunohistochemistry-Paraffin: DDHD2 Antibody [NBP1-82965] - Staining of human small intestine shows strong positivity in glandular cells.Applications for DDHD2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DDHD2
Alternate Names
DDHD domain containing 2, DDHD domain-containing protein 2, EC 3.1.1.-, KIAA0725PA-PLA1 like, phospholipase DDHD2, SAM, WWE and DDHD domain containing 1, SAM, WWE and DDHD domain-containing protein 1, SAMWD1, sec23p-interacting protein p125-like phosphatidic acid-preferring phospholipaseA1
Gene Symbol
DDHD2
Additional DDHD2 Products
Product Documents for DDHD2 Antibody
Product Specific Notices for DDHD2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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