DDIT4 Antibody (PS00-10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32259
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PS00-10
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full length protein. (Uniprot: Q9NX09)
Localization
Mitochondrion, Cytoplasm, cytosol.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DDIT4 Antibody (PS00-10)
Western Blot: DDIT4 Antibody (PS00-10) [NBP3-32259]
Western Blot: DDIT4 Antibody (PS00-10) [NBP3-32259] - Western blot analysis of DDIT4 on different lysates with Rabbit anti-DDIT4 antibody (NBP3-32259) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 25 kDa
Observed band size: 30 kDa
Exposure time: 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32259) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for DDIT4 Antibody (PS00-10)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DDIT4
Alternate Names
Dig2, DNA-damage-inducible transcript 4, FLJ20500, HIF-1 responsive RTP801 (RTP801), Protein regulated in development and DNA damage response 1, REDD-1DNA damage-inducible transcript 4 protein, REDD1HIF-1 responsive protein RTP801, RTP801RP11-442H21.1
Gene Symbol
DDIT4
Additional DDIT4 Products
Product Documents for DDIT4 Antibody (PS00-10)
Product Specific Notices for DDIT4 Antibody (PS00-10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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