Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Chimpanzee (100%), Equine (100%), Monkey (100%), Orangutan (100%), Primate (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DDIT4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human protein regulated in development and DNA damage response 1 (DNA-damage-inducible transcript 4) using the numbering given in entry NP_061931.1 (GeneID 54541).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25867045)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDIT4 Antibody

Western Blot: DDIT4 Antibody [NBP1-22966]

Western Blot: DDIT4 Antibody [NBP1-22966]

Western Blot: DDIT4 Antibody [NBP1-22966] - Influenza virus down-regulates REDD1 to promote mTORC1 activity. A549 cells were infected with WSN at MOI of 2 PFU/cell for the indicated times. A549 cells were transfected with siRNAs (pool of three each) targeting viral mRNAs and then infected for 7 h at MOI of 2 PFU/cell. Immunoblot analysis was performed to detect the depicted proteins, n = 3. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006635), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-22966]

Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody [NBP1-22966]

Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-22966] - HeLa cells stained with NBP1-22966 after 0.2% hypoxia or CoCl2 overnight. Image from verified customer review.
Western Blot: DDIT4 Antibody [NBP1-22966]

Western Blot: DDIT4 Antibody [NBP1-22966]

Western Blot: DDIT4 Antibody [NBP1-22966] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Lysate was prepared from cells that had been treated with cobalt chloride (A and B) or mock treated (A). NBP1-22966 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate.
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Applications for DDIT4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 to 1:2000

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:1000-1:4000
Application Notes
Formaldehyde fixation is recommended. Permeabilization with Triton X-100 is recommended for formaldehydefixed cells.

Reviewed Applications

Read 1 review rated 4 using NBP1-22966 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDIT4

REDD1 (regulated in development and DNA damage response 1) is a direct transcriptional target of HIF. It plays a role in the TSC/mTOR pathway as a regulator upstream of the TSC1/2 complex. In response to both hypoxia and energy stress, REDD1 inhibits mTOR activity in a TSC-dependent manner.

Alternate Names

Dig2, DNA-damage-inducible transcript 4, FLJ20500, HIF-1 responsive RTP801 (RTP801), Protein regulated in development and DNA damage response 1, REDD-1DNA damage-inducible transcript 4 protein, REDD1HIF-1 responsive protein RTP801, RTP801RP11-442H21.1

Entrez Gene IDs

54541 (Human)

Gene Symbol

DDIT4

UniProt

Q9NX09 (Human)

Additional DDIT4 Products

Product Documents for DDIT4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DDIT4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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