Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all DDIT4 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 12 amino acid synthetic peptide from near the center of human RTP801. The immunogen is located within amino acids 90 - 140 of RTP801. Amino Acid Squence: QARLGSRRPARL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDIT4 Antibody - BSA Free

Knockdown Validated: DDIT4 Antibody - BSA Free [NBP1-77321]

Knockdown Validated: DDIT4 Antibody - BSA Free [NBP1-77321]

Knockdown Validated: DDIT4 Antibody [NBP1-77321] - siRNA knockdown of the DDIT4 antiproliferative responses to 1,25(OH)2 D3RMCs were transfected with negative control siRNA orDDIT4-specific siRNA in the presence or absence of 1,25(OH) 2 D3 . (C) DDIT4 protein expression levels were detected by Western blot. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.23063) licensed under a CC-BY license.
Western Blot: DDIT4 AntibodyBSA Free [NBP1-77321]

Western Blot: DDIT4 AntibodyBSA Free [NBP1-77321]

Western Blot: DDIT4 Antibody [NBP1-77321] - 1,25(OH)2 D3 modulates RMCs proliferation induced by high glucose via the DDIT4/mTOR signaling pathwayRMCs were cultured for 48 h in the presence or absence of 1,25(OH)2 D3. Cell lysates were subjected to Western blot analysis to measure the levels of DDIT4. LG: 5.5 mM glucose; Man: 5.5 mM glucose + 24.5 mM mannitol; HG: 30 mM glucose. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.23063) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody - BSA Free [NBP1-77321]

Immunocytochemistry/ Immunofluorescence: DDIT4 Antibody - BSA Free [NBP1-77321]

Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP1-77321] - Mouse kidney tissue with NBP1-77321 antibody at 20 ug/mL. Red: Antibody Blue: DAPI staining
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Applications for DDIT4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDIT4

RTP801 was initially identified as a gene induced by DNA damage, and later found to also be regulated by other cellular stresses such as hypoxia and glucocorticoid treatment. Recently, RTP801 has been shown to act as a mediator of tuberous sclerosis complex (TSC)-dependent regulation of the mammalian Target of Rapamycin (mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle. In response to energy stress, RTP801 inhibits mTOR function, resulting in dephosphorylation of downstream targets such as ribosomal protein S6 kinase 1 and 4EBP1 and decreasing cell growth. Disregulation of RTP801 may thus contribute to human tumorigenesis.

Alternate Names

Dig2, DNA-damage-inducible transcript 4, FLJ20500, HIF-1 responsive RTP801 (RTP801), Protein regulated in development and DNA damage response 1, REDD-1DNA damage-inducible transcript 4 protein, REDD1HIF-1 responsive protein RTP801, RTP801RP11-442H21.1

Gene Symbol

DDIT4

UniProt

NP_061931 (Human)

Additional DDIT4 Products

Product Documents for DDIT4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DDIT4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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